7U4M
Crystal structure of human GPX4-U46C in complex with LOC1886
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-28 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.828, 115.654, 61.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.510 - 1.930 |
| R-factor | 0.1646 |
| Rwork | 0.161 |
| R-free | 0.19710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7l8k |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.220 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.126 | 2.577 |
| Number of reflections | 23683 | 1140 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.1 | 84.4 |
| Redundancy | 13.2 | 11.5 |
| CC(1/2) | 0.998 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 291 | 2 M sodium chloride and 0.1 M sodium acetate trihydrate |
