7U45
[L344] Self-assembling tensegrity triangle with three turns, four turns and four turns of DNA per axis by extension and linker addition with P1 symmetry
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00743 |
| Spacegroup name | P 1 |
| Unit cell lengths | 100.156, 135.049, 135.924 |
| Unit cell angles | 102.92, 96.91, 108.05 |
Refinement procedure
| Resolution | 54.050 - 8.050 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.894 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 93.214 | 93.214 | 9.155 |
| High resolution limit [Å] | 8.050 | 24.817 | 8.055 |
| Number of reflections | 3814 | 200 | 201 |
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 85.2 | ||
| Redundancy | 3.6 | 3.2 | 3.6 |
| CC(1/2) | 1.000 | 0.999 | 0.353 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 100 mM MOPS, 125 mM magnesium sulfate, sodium hydroxide |
