7U45

[L344] Self-assembling tensegrity triangle with three turns, four turns and four turns of DNA per axis by extension and linker addition with P1 symmetry

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 1
Unit cell lengths	100.156, 135.049, 135.924
Unit cell angles	102.92, 96.91, 108.05

Refinement procedure

Resolution	54.050 - 8.050
R-factor	0.235
Rwork	0.233
R-free	0.28520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.006
RMSD bond angle	0.894
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.214	93.214	9.155
High resolution limit [Å]	8.050	24.817	8.055
Number of reflections	3814	200	201
<I/σ(I)>	11.9
Completeness [%]	85.2
Redundancy	3.6	3.2	3.6
CC(1/2)	1.000	0.999	0.353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	100 mM MOPS, 125 mM magnesium sulfate, sodium hydroxide