7U40

[L244] Self-assembling tensegrity triangle with two turns, four turns and four turns of DNA per axis by linker addition with P1 symmetry

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 1
Unit cell lengths	67.638, 135.539, 136.111
Unit cell angles	100.06, 103.07, 96.36

Refinement procedure

Resolution	63.630 - 7.550
R-factor	0.1632
Rwork	0.161
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.006
RMSD bond angle	0.923
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.898	83.898	8.015
High resolution limit [Å]	7.330	22.130	7.338
Number of reflections	4126	205	206
<I/σ(I)>	4.8
Completeness [%]	83.6
Redundancy	3.6	3.3	3.7
CC(1/2)	0.999	0.998	0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	MOPS, Magnesium sulfate, Sodium hydroxide