7U3X

[F233] Self-assembling tensegrity triangle with two turns, three turns and three turns of DNA per axis by extension with P1 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-17
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 1
Unit cell lengths	67.961, 100.275, 102.065
Unit cell angles	100.58, 100.81, 102.39

Refinement procedure

Resolution	41.050 - 5.680
R-factor	0.1262
Rwork	0.124
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.033
RMSD bond angle	2.603
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	97.489	97.489	6.140
High resolution limit [Å]	5.680	17.156	5.680
Number of reflections	5122	255	257
<I/σ(I)>	5.1
Completeness [%]	87.1
Redundancy	3.6	3.7	3.7
CC(1/2)	0.998	0.998	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	MOPS, Magnesium sulfate, Sodium hydroxide