7U3V

[F224] Self-assembling tensegrity triangle with two turns, two turns and four turns of DNA per axis by extension with P1 symmetry

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-18
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 1
Unit cell lengths	67.750, 68.838, 132.117
Unit cell angles	77.86, 78.36, 83.26

Refinement procedure

Resolution	42.330 - 5.140
R-factor	0.1434
Rwork	0.141
R-free	0.17360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.008
RMSD bond angle	1.001
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.093	67.093	5.990
High resolution limit [Å]	5.130	16.768	5.130
Number of reflections	5031	252	253
<I/σ(I)>	11.4
Completeness [%]	82.6
Redundancy	3.6	3.7	3.7
CC(1/2)	1.000	0.999	0.245

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	MOPS, Magnesium sulfate, Sodium hydroxide