7U3A
Structure of the Streptomyces venezuelae GlgX-c-di-GMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-12 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.470, 127.660, 179.720 |
Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
Resolution | 89.670 - 3.340 |
R-factor | 0.2085 |
Rwork | 0.205 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7u39 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.670 | 3.420 |
High resolution limit [Å] | 3.340 | 3.340 |
Rpim | 0.095 | 0.228 |
Number of reflections | 42293 | 2854 |
<I/σ(I)> | 6.2 | 1.9 |
Completeness [%] | 99.5 | |
Redundancy | 2 | |
CC(1/2) | 0.977 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 40% ethylene glycol, 0.1 M sodium acetate pH 4.6 |