7U2S
Structure of Paenibacillus xerothermodurans Apyc1 in the apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-02-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.030, 58.295, 76.188 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 38.640 - 1.550 |
| R-factor | 0.1733 |
| Rwork | 0.173 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7u2r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.926 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.640 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rpim | 0.078 | 0.297 |
| Number of reflections | 74391 | 3615 |
| <I/σ(I)> | 5.4 | 2.3 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES-KOH 7.5, 0.2 M Calcium Acetate, 10% PEG-8,000 |
