7U2R
Structure of Paenibacillus sp. J14 Apyc1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-02-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 102.868, 102.868, 147.619 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.140 - 1.850 |
| R-factor | 0.1778 |
| Rwork | 0.177 |
| R-free | 0.19980 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.140 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rpim | 0.038 | 0.666 |
| Number of reflections | 39926 | 2401 |
| <I/σ(I)> | 12.1 | 1.2 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 11.3 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tric-HCl pH 8.5, 0.2 M MgCl2, 16% PEG-4000 |
