7U2H

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, and deacylated E-site tRNAgly at 2.55A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.610, 451.440, 624.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	146.300 - 2.550
R-factor	0.2218
Rwork	0.220
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	146.300	146.300	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.268	0.080	2.364
Rmeas	0.283	0.084	2.491
Total number of observations	19099952
Number of reflections	1896410	22338	140194
<I/σ(I)>	6.98	20.57	0.93
Completeness [%]	99.5	99.3	99.8
Redundancy	10.072	10.537	10.108
CC(1/2)	0.988	0.993	0.185

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME