7U1U

Crystal structure of arabidopsis thaliana acetohydroxyacid synthase W574L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979
Spacegroup name	P 64 2 2
Unit cell lengths	177.953, 177.953, 184.727
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.100 - 3.220
R-factor	0.187
Rwork	0.186
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k2o
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	3.420
High resolution limit [Å]	3.220	3.220
Number of reflections	28382	4412
<I/σ(I)>	10.5	2.3
Completeness [%]	99.5
Redundancy	10.6
CC(1/2)	0.997	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.4	293	CHES buffer, potassium sodium tartrate, sodium sulfate