7U1N

Crystal structure of the Anopheles darlingi AD-118 long form D7 salivary protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	CCD
Collection date	2020-08-12
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	70.730, 71.600, 71.750
Unit cell angles	90.00, 111.23, 90.00

Refinement procedure

Resolution	29.390 - 2.400
R-factor	0.2107
Rwork	0.206
R-free	0.26850
Structure solution method	MIRAS
RMSD bond length	0.009
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.390	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.065	0.284
Number of reflections	24809	1239
<I/σ(I)>	15.77
Completeness [%]	94.1	63.4
Redundancy	4.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	30%P EG 5000 MME, 0.2 M ammonium sulfate, 0.1M HEPES