7U0V

Structure of myxoma virus M062 protein variant Lau

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 64
Unit cell lengths	94.827, 94.827, 44.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.410 - 2.450
R-factor	0.2169
Rwork	0.211
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cyw
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.123	2.528
High resolution limit [Å]	2.450	2.450
Rmerge	0.054
Rmeas	0.057
Rpim	0.018
Number of reflections		846
<I/σ(I)>	23.92
Completeness [%]	99.5
Redundancy	9.8
CC(1/2)	0.990	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	50mM MgCl2 14% ethylene glycol 6% PEG8K 0.1M Tris pH 7.5