7U02

Structure of the C. crescentus DriD C-domain bound to ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.01
Spacegroup name	P 31 2 1
Unit cell lengths	87.400, 87.400, 139.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.540 - 2.480
R-factor	0.2338
Rwork	0.230
R-free	0.27900
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.600	2.490
High resolution limit [Å]	2.450	2.450
Rpim	0.033	1.034
Number of reflections	43538	1977
<I/σ(I)>	15	0.7
Completeness [%]	99.9
Redundancy	7.9
CC(1/2)	1.000	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	Ammonium sulphate