7U01

Structure of CD148 fibronectin type III domain 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979276
Spacegroup name	P 41 21 2
Unit cell lengths	155.068, 155.068, 47.837
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.711 - 2.297
R-factor	0.21719288275
Rwork	0.214
R-free	0.26042
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.591
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.354
High resolution limit [Å]	2.297	2.297
Rmerge	0.098	1.100
Number of reflections	26929	1712
<I/σ(I)>	22.4
Completeness [%]	100.0
Redundancy	26.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.1M sodium acetate pH4.5, 3M NaCl