7TZV

Structure of DriD C-domain bound to 9mer ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS EIGER2 S 4M
Wavelength(s)	1.01
Spacegroup name	P 21 21 21
Unit cell lengths	58.023, 85.338, 93.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.490 - 1.650
R-factor	0.1929
Rwork	0.192
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DriD Cdomain p3121 form
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.100	1.690
High resolution limit [Å]	1.650	1.650
Rpim	0.029	0.978
Number of reflections	55422	3432
<I/σ(I)>	16.1	1.2
Completeness [%]	98.1
Redundancy	5.9
CC(1/2)	0.999	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	0.1 M MES pH 6.5, 0.1 M Imidazole, 20% PEG 8000