7TZE

Structure of murine LAG3 domains 1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	35.729, 92.534, 81.771
Unit cell angles	90.00, 90.78, 90.00

Refinement procedure

Resolution	40.270 - 2.120
R-factor	0.2133
Rwork	0.212
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l1d 3b43
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1-3660_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.270	40.270	2.180
High resolution limit [Å]	2.050	6.130	2.050
Rmerge	0.085	0.041	1.328
Rmeas	0.099	0.048	1.534
Total number of observations	125635
Number of reflections	32665	1252	5142
<I/σ(I)>	8.28	27.21	0.93
Completeness [%]	98.1	97.5	96.3
Redundancy	3.846	3.565	3.976
CC(1/2)	0.997	0.998	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2 M ammonium sulfate