7TZ2

Structure of human Fibrinogen-like protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-01
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9791
Spacegroup name	P 2 21 21
Unit cell lengths	45.277, 110.893, 148.888
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.300 - 2.550
R-factor	0.1871
Rwork	0.185
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m7f
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.550	5.490	2.550
Rmerge	0.349	0.180	1.347
Rmeas	0.384	0.199	1.518
Rpim	0.157	0.083	0.686
Total number of observations	144191
Number of reflections	25222	2723	2457
<I/σ(I)>	3
Completeness [%]	99.7	99.5	99.4
Redundancy	5.7	5.7	4.5
CC(1/2)		0.901	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1 M lithium chloride, 0.1 M citric acid pH 5.0 and 20% PEG 6000