7TYG

Structure of the human leucine rich repeat protein SHOC2, residues 80-582

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	91.350, 102.880, 120.830
Unit cell angles	90.00, 101.74, 90.00

Refinement procedure

Resolution	41.440 - 1.900
R-factor	0.1898
Rwork	0.188
R-free	0.22580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tzh
RMSD bond length	0.007
RMSD bond angle	0.858
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER (1.18.2_3874+SVN)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	118.303	1.902
High resolution limit [Å]	1.895	1.895
Rmerge	0.051	0.539
Rmeas	0.061	0.644
Number of reflections	82107	869
<I/σ(I)>	13	2
Completeness [%]	94.7	100
Redundancy	3.3	3.3
CC(1/2)	0.998	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	0.1M Tris-Cl(8.0), 0.2 M MgCl2, 11% PEG4K, 2.5% 1,5-diaminopentane