7TXA
Structure of the Class II Fructose-1,6-Bisphophatase from Francisella tularensis complexed with native metal cofactor Mn++ and product F6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.394, 76.628, 141.465 |
| Unit cell angles | 76.84, 87.23, 75.71 |
Refinement procedure
| Resolution | 39.100 - 2.400 |
| R-factor | 0.1984 |
| Rwork | 0.196 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7js3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.198 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.043 | 0.390 |
| Rmeas | 0.075 | 0.550 |
| Rpim | 0.042 | 0.320 |
| Number of reflections | 101338 | 9973 |
| <I/σ(I)> | 17.55 | 2 |
| Completeness [%] | 98.4 | 97.8 |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.995 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2 M sodium malonate pH 6.0, 20% PEG 3350 |
