7TVP

Viral AMG chitosanase V-Csn, E157Q mutant, chitotriose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	108.939, 47.421, 45.402
Unit cell angles	90.00, 97.83, 90.00

Refinement procedure

Resolution	37.130 - 1.300
R-factor	0.1335
Rwork	0.132
R-free	0.16400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tvl
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.130	1.320
High resolution limit [Å]	1.300	1.300
Rmeas	0.090	0.964
Rpim	0.034	0.380
Number of reflections	55448	2455
<I/σ(I)>	11.7	1.8
Completeness [%]	97.8	87.9
Redundancy	6.7
CC(1/2)	0.999	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME