7TVO

Viral AMG chitosanase V-Csn, E157Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	108.584, 47.434, 45.460
Unit cell angles	90.00, 97.89, 90.00

Refinement procedure

Resolution	37.120 - 1.150
R-factor	0.1193
Rwork	0.118
R-free	0.13500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tvl
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.120	1.170
High resolution limit [Å]	1.150	1.150
Rmeas	0.050	0.361
Rpim	0.019	0.135
Number of reflections	76188	3552
<I/σ(I)>	22.1	5.1
Completeness [%]	94.0	88.5
Redundancy	7.2
CC(1/2)	1.000	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME