7TVN

Viral AMG chitosanase V-Csn, D148N mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	108.760, 47.450, 45.431
Unit cell angles	90.00, 97.96, 90.00

Refinement procedure

Resolution	37.150 - 1.200
R-factor	0.1263
Rwork	0.125
R-free	0.14890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tvl
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.200	1.220
High resolution limit [Å]	1.200	1.200
Rmeas	0.071	0.702
Rpim	0.027	0.285
Number of reflections	70261	3137
<I/σ(I)>	16.1	2.6
Completeness [%]	98.1	88.4
Redundancy	6.7
CC(1/2)	0.999	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME