7TVL

Viral AMG chitosanase V-Csn, apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.72929
Spacegroup name	C 1 2 1
Unit cell lengths	108.836, 47.632, 45.546
Unit cell angles	90.00, 97.82, 90.00

Refinement procedure

Resolution	37.180 - 0.890
R-factor	0.1203
Rwork	0.120
R-free	0.13070
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.200	0.910
High resolution limit [Å]	0.890	0.890
Rmeas	0.093
Rpim	0.036	0.549
Number of reflections	174574	7896
<I/σ(I)>	11.4
Completeness [%]	99.1
Redundancy	6.6
CC(1/2)	0.998	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 30% PEG2000 MME