7TVJ

Crystal Structure of Monobody Mb(SHP2PTP_13)/SHP2 PTP Domain Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	48.460, 48.490, 102.070
Unit cell angles	80.14, 77.53, 61.63

Refinement procedure

Resolution	49.670 - 2.390
R-factor	0.2306
Rwork	0.229
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3ZM1 & 3K2M
RMSD bond length	0.002
RMSD bond angle	0.535
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.670	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.084	0.245
Rmeas	0.118	0.347
Rpim	0.084	0.245
Number of reflections	29422	3038
<I/σ(I)>	6.7	2.3
Completeness [%]	93.3	92.1
Redundancy	1.8	1.7
CC(1/2)	0.974	0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	292	0.1 M sodium citrate tribasic dihydrate, 2% Tascimate, 16% PEG3350