7TVB

Stat5A Core in Complex with AK305

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1271
Spacegroup name	H 3 2
Unit cell lengths	239.937, 239.937, 113.224
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.990 - 2.653
R-factor	0.2107
Rwork	0.209
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Previously solved structure in lab
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (20-OCT-2021))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.650	7.190	2.650
Rmerge	0.095	0.032	0.976
Rmeas	0.097	0.033	1.005
Rpim	0.021	0.007	0.237
Total number of observations	741459
Number of reflections	36049	1875	1795
<I/σ(I)>	6.3
Completeness [%]	99.9	100	99.9
Redundancy	20.6	21.5	17.2
CC(1/2)		1.000	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1M Na Malonate pH 7.0, 1% Jeffamine pH 7.0, 5% peg 400