7TUV

Crystal structure of the exoribonucleolytic module of T. brucei RRP44

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	66.296, 91.113, 148.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.630 - 2.225
R-factor	0.1977
Rwork	0.194
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology model
RMSD bond length	0.009
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.632	2.554
High resolution limit [Å]	2.225	2.225
Number of reflections	24678	1235
<I/σ(I)>	12.1
Completeness [%]	93.8	70.6
Redundancy	24
CC(1/2)	0.998	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	15% (w/v) PEG 20000, 0.1 M MES pH 6.0