7TU2
Structure of the L. blandensis dGTPase R37A mutant bound to Mn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 179.934, 179.934, 110.941 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.220 - 2.130 |
R-factor | 0.2046 |
Rwork | 0.204 |
R-free | 0.22590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bg2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.021 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.250 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.118 | 1.763 |
Rmeas | 0.100 | 1.869 |
Number of reflections | 102014 | 16196 |
<I/σ(I)> | 9.96 | 1.15 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 9.4 | 8.9 |
CC(1/2) | 0.997 | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Crystal grown in 1ul:1ul drops of well solution (0.6M Sodium Potassium Tartrate, 0.2M Lithium Sulfate, 0.1M Bis-Tris pH 6.5) and 10mg/mL protein. Cryoprotected by soaking in well solution with 30% ethylene glycol and 10mM MnCl2 added |