7TTY

Crystal structure of potent neutralizing antibody 10-40 in complex with bat WIV1 receptor-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	110
Detector technology	PIXEL
Collection date	2021-12-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979180
Spacegroup name	P 1 21 1
Unit cell lengths	51.311, 69.546, 95.215
Unit cell angles	90.00, 105.63, 90.00

Refinement procedure

Resolution	91.690 - 3.110
R-factor	0.244
Rwork	0.235
R-free	0.32930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7sd5
RMSD bond length	0.015
RMSD bond angle	1.917
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.690	10.000	3.410
High resolution limit [Å]	3.110	8.000	3.190
Rmerge	0.190	0.257	1.790
Rmeas	0.400	0.279	0.900
Rpim	0.165	0.107	0.801
Number of reflections	11493	524	1930
<I/σ(I)>	2
Completeness [%]	99.6	99.9	99.8
Redundancy	6.8	6.6	6.9
CC(1/2)	0.943	0.956	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M sodium citrate, pH 5.5, 20% propanol, 20% PEG4000