7TTX

Crystal structure of potent neutralizing antibody 10-40 in complex with Sarbecovirus bat RaTG13 receptor-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	110
Detector technology	PIXEL
Collection date	2021-07-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979110
Spacegroup name	P 21 21 21
Unit cell lengths	74.174, 76.816, 117.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.320 - 2.800
R-factor	0.2269
Rwork	0.222
R-free	0.32110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7sd5
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	117.630	117.630	2.800
High resolution limit [Å]	2.670	8.850	2.670
Rmerge	0.173	0.087	2.259
Rmeas	0.180	0.091	2.361
Rpim	0.050	0.026	0.669
Total number of observations	249834	6799	28449
Number of reflections	19519	614	2433
<I/σ(I)>	8.8	25.1	0.9
Completeness [%]	98.9	98.2	94.5
Redundancy	12.8	11.1	11.7
CC(1/2)	0.997	0.996	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1 M lithium chloride, 30% w/v PEG6000, 0.1 M sodium acetate