7TSW
Crystal Structure Of Human NADH-Cytochrome B5 Reductase T117D Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-27 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.541780 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.562, 79.986, 172.358 |
| Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
| Resolution | 38.660 - 2.400 |
| R-factor | 0.2276 |
| Rwork | 0.227 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.030 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.111 | 0.471 |
| Rmeas | 0.120 | 0.500 |
| Number of reflections | 80627 | 7363 |
| <I/σ(I)> | 8.7 | 3 |
| Completeness [%] | 94.0 | 86.8 |
| Redundancy | 7.71 | 8.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | PEG 4K 10%, 0.1M Tris-HCl pH 8.4 |
