7TRO

Crystal structure of R14A-R20A human Galectin-7 mutant in presence of lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-19
Detector	RAYONIX MX300HE
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	53.970, 66.160, 70.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.970 - 1.800
R-factor	0.178
Rwork	0.175
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gal
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.12)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.280	48.280	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.052	0.028	0.793
Rmeas	0.055	0.030	0.861
Rpim	0.018	0.010	0.328
Number of reflections	23994	2550	2367
<I/σ(I)>	24.7	59.4	2.2
Completeness [%]	99.8	99.8	99.8
Redundancy	9.3	9.2	6.6
CC(1/2)	1.000	1.000	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295.15	0.1 M NaCl,0.1 M Tris pH 8, 20 % PEG 6000, 5% Glycerol, 7.5 mM Lactose