7TRO
Crystal structure of R14A-R20A human Galectin-7 mutant in presence of lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.970, 66.160, 70.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.970 - 1.800 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gal |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.12) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.280 | 48.280 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.052 | 0.028 | 0.793 |
Rmeas | 0.055 | 0.030 | 0.861 |
Rpim | 0.018 | 0.010 | 0.328 |
Number of reflections | 23994 | 2550 | 2367 |
<I/σ(I)> | 24.7 | 59.4 | 2.2 |
Completeness [%] | 99.8 | 99.8 | 99.8 |
Redundancy | 9.3 | 9.2 | 6.6 |
CC(1/2) | 1.000 | 1.000 | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.1 M NaCl,0.1 M Tris pH 8, 20 % PEG 6000, 5% Glycerol, 7.5 mM Lactose |