7TR7

APE1 product complex with abasic ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-15
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	83.717, 83.717, 204.179
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.810 - 2.000
R-factor	0.1834
Rwork	0.182
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dff
RMSD bond length	0.011
RMSD bond angle	1.088
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.030
High resolution limit [Å]	2.000	2.000
Rmeas	0.080
Number of reflections	83056	2717
<I/σ(I)>	20.8
Completeness [%]	100.0	99.9
Redundancy	6.3	4.3
CC(1/2)		0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.05M KCl, 0.05M sodium cacodylate pH 6, 10% PEG 8,000, 5mM spermine, 5mM L-Argininamide dihydrochloride