7TQQ
Structure of human TREX1-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-02-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.681, 91.356, 157.386 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 45.680 - 2.200 |
| R-factor | 0.2274 |
| Rwork | 0.226 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mxj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.789 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.680 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.085 | 0.766 |
| Number of reflections | 62516 | 4576 |
| <I/σ(I)> | 5.6 | 1.1 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 5.5 | 5.3 |
| CC(1/2) | 0.990 | 0.325 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05 M Li2SO4, 0.1 M Bis-Tris pH 6.5, 25% PEG-3350 |
