7TQP

Structure of human TREX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	57.582, 57.582, 125.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.470 - 1.950
R-factor	0.207
Rwork	0.204
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mxj
RMSD bond length	0.002
RMSD bond angle	0.580
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.470	2.000
High resolution limit [Å]	1.950	1.950
Rpim	0.049
Number of reflections	16216	1098
<I/σ(I)>	9.7	1.2
Completeness [%]	99.9
Redundancy	21.6
CC(1/2)	0.998	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M NaCl, 0.1 M CHES pH 9.5, 1.26 M (NH4)2SO4