7TQO

Structure of human TREX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	57.592, 57.592, 124.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.260 - 1.250
R-factor	0.1719
Rwork	0.171
R-free	0.18950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mxj
RMSD bond length	0.007
RMSD bond angle	0.942
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.260	1.270
High resolution limit [Å]	1.250	1.250
Rpim	0.017
Number of reflections	58943	2773
<I/σ(I)>	19	1.2
Completeness [%]	99.8
Redundancy	45.6
CC(1/2)	1.000	0.449

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES-KOH pH 7.6, 10% PEG-8000