7TQB

Crystal structure of monoclonal S9.6 Fab bound to DNA-RNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	38.426, 158.884, 43.142
Unit cell angles	90.00, 105.15, 90.00

Refinement procedure

Resolution	37.091 - 3.100
R-factor	0.2317
Rwork	0.229
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tt1
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.500	3.130
High resolution limit [Å]	3.020	3.020
Number of reflections	9509	925
<I/σ(I)>	3.78	1.55
Completeness [%]	98.7
Redundancy	12.1
CC(1/2)	0.841	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG 1000 0.2 M sodium narrate