7TNI
Structure of EC12 Y1392W variant of BT-R1 from Manduca sexta, a Cry1A toxin binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979110 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.133, 44.579, 94.797 |
| Unit cell angles | 90.00, 107.16, 90.00 |
Refinement procedure
| Resolution | 56.500 - 1.900 |
| Rwork | 0.210 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ee0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.765 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.500 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.837 |
| Rmeas | 0.078 | 0.922 |
| Rpim | 0.032 | 0.382 |
| Number of reflections | 37385 | 2417 |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 5.5 | 5.6 |
| CC(1/2) | 0.998 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M Tris-HCl pH 7.0, 2.8M Magnesium Sulfate |
