7TL4

Crystal Structure of Yeast p58C Multi-Tyrosine Mutant 6YF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-06
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	39.519, 51.488, 90.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.606 - 1.805
R-factor	0.1971
Rwork	0.196
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dtz
RMSD bond length	0.016
RMSD bond angle	1.253
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.085	0.064	0.338
Rmeas	0.095	0.071	0.381
Rpim	0.041	0.031	0.172
Total number of observations	90999
Number of reflections	16970	1798	1489
<I/σ(I)>	13.7
Completeness [%]	97.0	94.3	87.5
Redundancy	5.4	5.2	4.4
CC(1/2)		0.990	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Protein drops contained 20 mM HEPES (pH 6.8), 75 mM NaCl, 2 mM DTT mixed with equal volume of 100 mM TRIS (pH 8.5) and 55-70% MPD.