7TKZ

Crystal structure of D94A human Galectin-7 mutant in presence of lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	30.230, 76.400, 111.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.930 - 1.830
R-factor	0.1693
Rwork	0.166
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gal
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.12)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.940	44.940	1.900
High resolution limit [Å]	1.830	3.940	1.830
Rmerge	0.074	0.054	0.248
Rmeas	0.081	0.059	0.271
Rpim	0.107	0.024	0.107
Number of reflections	23542	2530	2344
<I/σ(I)>	6.8	28.3	6.8
Completeness [%]	99.9	99.8	99.9
Redundancy	6.2	6	6.3
CC(1/2)	0.997	0.997	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.1 M NaCl, 0.1 M Tris pH 8.0, 20 % PEG 6000, 15% glycerol, 7.5 mM Lactose