7TKX
Crystal structure of R14A human Galectin-7 mutant in presence of 4-O-beta-D-Galactopyranosyl-D-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.250, 76.380, 111.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.120 - 1.830 |
| R-factor | 0.1716 |
| Rwork | 0.169 |
| R-free | 0.21340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gal |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.289 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.7.12) |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.120 | 33.380 | 1.900 |
| High resolution limit [Å] | 1.830 | 3.940 | 1.830 |
| Rmerge | 0.078 | 0.038 | 0.749 |
| Rmeas | 0.085 | 0.041 | 0.805 |
| Rpim | 0.032 | 0.016 | 0.294 |
| Number of reflections | 23270 | 2534 | 2241 |
| <I/σ(I)> | 17.5 | 39 | 3 |
| Completeness [%] | 98.6 | 99.6 | 95.8 |
| Redundancy | 7.2 | 6.7 | 7.4 |
| CC(1/2) | 0.998 | 0.998 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 10% Glycerol |
