7TJO

HIV-1 gp120 complex with CJF-II-197-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.339, 121.826, 195.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 3.070
R-factor	0.2326
Rwork	0.229
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tgr
RMSD bond length	0.004
RMSD bond angle	0.683
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.350
High resolution limit [Å]	3.070	3.070
Rmerge	0.283
Number of reflections	23992	1200
<I/σ(I)>	8.4
Completeness [%]	99.8
Redundancy	13.4
CC(1/2)	0.996	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14%(w/v) PEG 1500, 0.1 M calcium chloride, 0.1 M imidazole pH 6.5