7TGQ

Zinc finger antiviral protein (ZAP) central domain bound to ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 3 2 1
Unit cell lengths	89.958, 89.958, 52.807
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.710 - 2.000
R-factor	0.2399
Rwork	0.238
R-free	0.27280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kzh
RMSD bond length	0.014
RMSD bond angle	1.039
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.111	1.000
Rmeas	0.114	1.000
Rpim	0.025	0.592
Number of reflections	17745	875
<I/σ(I)>	25.2	1.1
Completeness [%]	100.0
Redundancy	21.2
CC(1/2)	0.987	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8-1.1 M sodium nitrate, 0.1 M sodium acetate