7TGI
Single-domain VHH intrabodies neutralize ricin toxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-18 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 44.129, 84.407, 109.945 |
| Unit cell angles | 90.00, 97.06, 90.00 |
Refinement procedure
| Resolution | 42.203 - 2.104 |
| R-factor | 0.1784 |
| Rwork | 0.176 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtc 4lgr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.091 | 0.063 | 0.682 |
| Rmeas | 0.109 | 0.077 | 0.838 |
| Rpim | 0.059 | 0.042 | 0.480 |
| Total number of observations | 74897 | ||
| Number of reflections | 22929 | 1193 | 1087 |
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 98.7 | 98.6 | 97 |
| Redundancy | 3.3 | 3.2 | 2.8 |
| CC(1/2) | 0.993 | 0.586 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 200 mM magnesium chloride, 20% PEG 3350 |
