7TFM

Atomic Structure of the Leishmania spp. Hsp100 N-Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	60.971, 60.971, 71.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.963 - 1.055
R-factor	0.1345
Rwork	0.134
R-free	0.15280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wbw
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.070
High resolution limit [Å]	1.050	1.050
Rmeas		0.591
Rpim	0.023	0.259
Number of reflections	61983	2900
<I/σ(I)>	10.9
Completeness [%]	97.1
Redundancy	9.1
CC(1/2)		0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	287	5% PEG 2000, 5% PEG 3350, 5% PEG 4000, 5% PEGME 5000, 0.1 M KCl, 0.1 M MgCl2, 0.1 M PIPES pH 7.0