7TEC

Structure of the Listeria monocytogenes GlnR-DNA complex to 3.45 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.09
Spacegroup name	P 2 2 21
Unit cell lengths	28.632, 47.717, 121.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.510 - 3.450
R-factor	0.2701
Rwork	0.266
R-free	0.30910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tea
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.510	4.340
High resolution limit [Å]	3.450	3.450
Rpim	0.038	0.240
Number of reflections	2384	237
<I/σ(I)>	8.9	2
Completeness [%]	96.8
Redundancy	4.1
CC(1/2)	0.998	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		275	50 mM sodium cacodylate pH 6.5, 35% MPD, 10 mM MgCl2