7TE3

Crystal Structure of a Double Loop Deletion Mutant in gC1qR/C1qBP/HABP-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 63 2 2
Unit cell lengths	80.529, 80.529, 114.552
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.990 - 2.200
R-factor	0.2384
Rwork	0.233
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p32
RMSD bond length	0.007
RMSD bond angle	0.882
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (v1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.143	0.040	1.865
Rmeas	0.147	0.041	1.936
Rpim	0.032	0.009	0.499
Total number of observations	239844
Number of reflections	11799	1324	1122
<I/σ(I)>	6
Completeness [%]	99.9	99.6	99.6
Redundancy	20.3	19.4	13.8
CC(1/2)		1.000	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M BisTris-HCl pH 6.5, 0.1 M sodium chloride, 1.5 M ammonium sulfate