Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9774 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.197, 57.800, 66.440 |
| Unit cell angles | 90.00, 97.66, 90.00 |
Refinement procedure
| Resolution | 49.745 - 2.604 |
| Rwork | 0.217 |
| R-free | 0.27660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3emn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.542 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.745 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 11349 | 1290 |
| <I/σ(I)> | 18.7 | 2.7 |
| Completeness [%] | 99.0 | |
| Redundancy | 9.8 | |
| CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris-HCl, pH 8.5, 10% Polyethylene glycol (PEG) 400 and 16% 2-Methyl-2,4-pentanediol (MPD) |
