7TCS
M379A mutant tyrosine phenol-lyase complexed with L-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.550, 82.990, 96.580 |
| Unit cell angles | 93.73, 105.71, 114.24 |
Refinement procedure
| Resolution | 45.270 - 1.370 |
| R-factor | 0.1413 |
| Rwork | 0.141 |
| R-free | 0.15490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vlh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.905 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4444) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.870 | 1.346 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 363833 | 18122 |
| <I/σ(I)> | 10.46 | |
| Completeness [%] | 87.1 | |
| Redundancy | 7.8 | |
| CC(1/2) | 0.998 | 0.274 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, pH 7.5, 0.2 mM PLP, 1 mM EDTA, 1 mM DTT, 19% PEG 5000 MME, 0.4 M CsCl |
