7TCB
Crystal Structure of the YaeQ Family Protein VPA0551 from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.024, 47.687, 115.519 |
| Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
| Resolution | 40.920 - 2.700 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.26470 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.439 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.124 | 0.799 |
| Number of reflections | 24176 | 844 |
| <I/σ(I)> | 15.5 | 1.3 |
| Completeness [%] | 95.1 | 67.8 |
| Redundancy | 4.6 | 3.5 |
| CC(1/2) | 0.986 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25 % (w/v) PEG 3350 |
