7TBQ
LOV2-DARPIN fusion : D7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 93.212, 93.212, 233.136 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.140 - 2.678 |
| R-factor | 0.2418 |
| Rwork | 0.239 |
| R-free | 0.29490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tbn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.720 | 2.820 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.105 | |
| Rmeas | 0.110 | |
| Rpim | 0.034 | |
| Number of reflections | 56150 | 2813 |
| <I/σ(I)> | 12.8 | 1.5 |
| Completeness [%] | 94.0 | 48.2 |
| Redundancy | 10.1 | 10.2 |
| CC(1/2) | 0.999 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM sodium chloride, 25% PEG3350, 100 mM Tris, pH 5.5 |
